Eliosin-an alternative product from the HmPKD1 locus is a component of endoplasmic reticulum mitochondria membrane contact sites
Fig 5
Eliosin is expressed in human cells treated with PC1 siRNA.
Immunoblot of HK2 cells monitored for endogenous Eliosin expression was probed with anti-Polycystin C-terminal NM005 (Top panel). In lanes 1, 2, HK-2 cells were treated with scrambled siRNA or with 3 different siRNAs that bind to sequences located at the 5’ end of the HmPKD1 gene: siRNA-1, lanes 3,4; siRNA-2, lanes 5,6; siRNA-3, lane 7. A band of ~45-47 kDa corresponding to Eliosin and a high molecular weight band likely the full-length PC1 were detected in the scrambled siRNA treated cells (lanes 1, 2). The specific PKD1 siRNAs suppressed full-length PC1 whereas the expected band for Eliosin is unaffected. The ~ 68 kDa band (*) may be an Eliosin heteromer or non-specific background band. Lower image: Same immunoblot from the upper panel probed with anti-actin.