Eliosin-an alternative product from the HmPKD1 locus is a component of endoplasmic reticulum mitochondria membrane contact sites
Fig 2
Sequence and detection of human PKD1-208 alternative transcript.
A. Nucleotide sequence of wild type PKD1 transcript, the red boxed nucleotides delineate exon 42 which is deleted in alternative PKD1 transcript (PKD1-208). Bold sequences are primer sites. Each exon has been color coded. B. Pairwise alignment of nucleotide region for alternative PKD1 transcript and wild type PKD1, showing missing exon 42 in the alternative transcript. C. Schematic diagram of intronic and exon sequences from full-length HmPKD1 starting from intron 40. PKD1-208 transcript has an exon 42 skipping (triangle below exon). PCR primers used in this study are mapped to the diagram (nucleotide sequences are found in Table 1). D. Detection of wild type and alternative PKD1 transcript in human kidney tissue using common PKD1 primers (Listed on the left of the agarose gel image) (lane 1) and specific primers (lane 2 and 3) for the alternative transcript. M, 1Kb DNA ladder. E. Sequence of PKD1-208 cDNA with same color code as in A. Underlined nucleotide sequences represent protein coding region of alternative PKD1 transcript. Bold sequence CTG are potential Leucine translation start sites.