Multimodal analysis investigating the shared pathogenic mechanisms of osteoporosis and osteoarthritis with an initial exploration of the role of ferroptosis
Fig 5
Elevation of ferroptosis levels in BMSCs during OP progression.
(A)The micro-CT results of mouse bone tissue and (B) Bone volume over tissue volume (BV/TV), number of trabeculae (Tb.N), bone trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp) were determined. (C) H&E staining results of mouse bone tissue; (D) Flow cytometry analysis of BMSCs apoptosis levels; (E) Statistical analysis of (D); Levels of (F) Fe2+, (G) MDA, and (H) GSH in BMSCs; (H) Levels of SLC2A3, TXNIP, ACSL4, GPX4, and FTH1 in BMSCs; (I) Statistical analysis of (H).