Investigating the anti-osteosarcoma effects of Patchouli alcohol through protein network mapping and in vitro experiments
Fig 5
PA-induced apoptosis in osteosarcoma cells.
(A)The ratio of apoptosis in osteosarcoma cells was detected by Annexin V-FITC double staining combined with flow cytometry analysis. (B) Intracellular MPP turnover was detected by staining with probe JC-1 combined with flow cytometry analysis. (C) The expression levels of apoptosis-related proteins Bcl-2 and Bax in osteosarcoma cells (HOS and 143B) were measured by Western blot analysis. *p < 0.05 vs. control group.