Transcriptional regulation in skeletal muscle and adipose tissue of lean and obese colony cats
Fig 5
Cluster Heat Map of muscle tissue samples from cats sampled at two different time points and expressing varying genetic predisposition to obesity. n = 6 cats (3 cats without genetic predisposition to obesity (leanGL1, GL2, GL3), 3 cats with genetic predisposition to obesity (obese, GO1, GO2, GO3); all cats belonged to European Shorthair cats from an experimental cat colony.
T1 = Timepoint 1 (all cats lean (BCS ≤ 5.5) after energy restricted feeding; T2 = Timepoint 2 (Cats segregated into three lean (BCS ≤ 5.5) and three obese cats (BCS ≥ 6) after ad libitum feeding from T1 on. Genetic predisposition of half of the study cats was induced by crossbreeding. “Contamination” represents an unexpected subcluster which, according to annotation, represents a contamination of adipose tissue with non-adipose tissue. Based on the expression pattern it was concluded the non-adipose tissue to be muscle tissue from T1.