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A framework to enhance the signal-to-noise ratio for quantitative fluorescence microscopy

Fig 1

Illustrating the signal-to-noise ratio model of an EMCCD camera.

(A) The path of photons (green) emitted by the light source, arriving at the camera sensor, get converted into electrons (-) that are amplified and converted into pixel intensity by the Analogue-to-Digital converter (ADC). (B) The raw intensity at high and low signal to noise ratios showing the signal of interest relative to the noise.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0330718.g001