Detection of Fusarium solani using cutinase antibody and its application in diagnosing fungal keratitis in an animal model
Fig 5
Detection of Fusarium antigens in FK mouse model samples using cutinase antibody.
Fungal keratitis was induced in BALB/c mice by exposing the scratched cornea of each mouse to contact lenses infected with F. solani for 10 days. Compared to naïve mice, typical inflammation and symptoms of keratitis were observed in Fusarium-infected mice (A). Fusarium antigens were detected by ELISA assay using cutinase-specific antibodies in both tear-wash samples (B) and sonicated eyeballs in PBS (C). Negative controls (□) correspond to naïve tear-wash samples, and positive groups (■) correspond to FK-mouse tear-wash samples. Similarly, negative controls (○) correspond to naïve eyeball lysates, and positive groups (●) correspond to FK-mouse eyeball lysates. Asterisks indicate statistical significance between the means (*** P < 0.001 and **** P < 0.0001).