Strategies for improved endothelial cell adhesion in microphysiological vascular model systems
Fig 2
Design and schematic of the TEBV Chambers and microscope holders.
(A) i) Side view of the TEBV viewing chamber top. The outer recessed area is optimized to accommodate the front lens assembly housing, while the inner recessed area is designed to facilitate the observation of 4 TEBVs in the chamber; ii) Design and position of TEBV viewing chamber holders. The TEBV chamber is designed to be placed on top of 3D-printed viewing chamber holders. The outer dimensions of the holder are compatible with the slide holder on the microscopy stage, facilitating movement of the chamber during imaging. A central cutout in the holder allows for imaging of all four TEBVs within the chamber. (B) Schematic for the TEBV viewing chamber with associated components. The viewing chamber top features a square void designed to accommodate a thin glass slide (part 2, dimensions: 17.5 mm 19 mm) for direct visualization. The core chamber component (part 5) accommodates four TEBVs. Positioned at the chamber corners are four pedicle screws and nuts (part 1 and 8), while O-rings (part 3) are inserted into the grooves to ensure a secure seal. The two side ports (part 4) are tailored for side loop perfusion. In experiments where imaging capabilities are unnecessary, the viewing chamber top can be substituted with the same piece used for the bottom of the chamber (part 7). Figure created in Biorender (https://BioRender.com).