Correlation of p53 oligomeric status and its subcellular localization in the presence of the AML-associated NPM mutant
Fig 5
FCCS measurements in the nucleus of HEK-293T cells co-transfected with a mix of mVenus- and mRFP1-labeled p53 variants.
A) The cross-correlation amount Q was determined for each FCCS measurement. B) The diffusion time τD was calculated from autocorrelation curves recorded in the mVenus detection channel. Mean values are plotted with ±SD (left), red symbols in the 95% confidence graph (right) mark significant differences between appropriate pairs of samples (p < 0.05).