Small-molecule inhibitors of 6-phosphofructo-1-kinase simultaneously suppress lactate and superoxide generation in cancer cells
Fig 7
The influence of selected PFK1 compounds on glycolytic and respiratory parameters in Jurkat cells.
Respiratory fluxes, maximal respiration rates, glycolytic rates, and glycolytic capacities were measured in Jurkat cells in the presence and absence of selected cmpd. Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), measured in the first 20 minutes of the experiment, were significantly higher in the untreated cells (vehicle) than in the treated cells. For cmpd No. 9, the statistical significances between untreated and treated cells were P** < 0.01 for respiration and P*** < 0.005 for glycolytic rate, while for cmpd No. 30, significances of P** < 0.01 for respiration and P*** < 0.005 for glycolytic rate, were determined. After 20 minutes, oligomycin A and FCCP were added, which made it possible to evaluate the maximal respiration and glycolytic capacities. In Jurkat cells treated with cmpd No. 9, the highest values of < P* 0,05 were obtained for maximal respiration and P*** value <0.005 for glycolytic capacity, compared to the vehicle while in the cells treated with cmpd No. 30, the values for maximal respiration were P** < 0.01 and for glycolytic capacity P*** < 0.005, compared to the vehicle. Data represents three independent measurements expressed as mean ±SD (n = 3).