Small-molecule inhibitors of 6-phosphofructo-1-kinase simultaneously suppress lactate and superoxide generation in cancer cells
Fig 6
(a) Lactate suppression by sequential re-insertion of cmpd at low concentration in Jurkat cells.
Lactate excretion in tumorigenic Jurkat cells remained effectively reduced when the cmpd No. 9 and 30) were added periodically every 24 hours at low concentrations (10, 15, and 20 µM). At 72 hours of incubation, the values of statistically significant differences between the vehicle and the cells treated with 10 µM of No. 9 and No. 30 were P < 0.0005, respectively. Data represents three independent measurements expressed as mean ±SD (n = 3). (b) Superoxide (SOX) and (ROS) suppression by sequential re-insertion of cmpds at low concentrations in Jurkat cells. Suppressed ROS and SOX formation was observed in Jurkat cells when cmpds No. 9 or 30 were sequentially added to the medium at low concentrations (5, 10, and 15 µM). Although some suppression of SOX formation was observed at 5 µM of both camps, significant differences were observed between the vehicle and cells treated with both cmpds (10 and 15 µM). The dose-dependent effects of the different concentrations of cmpds No. 9 and 30 were also examined regarding the suppression of ROS formation. However, only SOX formation in Jurkat cells appeared more potently suppressed. However, at 72 hours of inoculation, the cmpds tested for suppression reactive oxygen species (ROS) proved less efficient in reaching the P values of < 0.1, compared to the vehicle. Under identical growth conditions, the statistically significant values of SOX detected by 10 µM concentrations were P < 0.005 for No. 9 and P < 0.005 for No. 30, compared to the vehicle. The data are representative of three independent measurements and are given as mean ±SD (n = 3).