Small-molecule inhibitors of 6-phosphofructo-1-kinase simultaneously suppress lactate and superoxide generation in cancer cells
Fig 5
Dose-dependent reduction of lactate formation by Jurkat cells.
Dose-dependent effects of cmpds No. 9 and 30 on lactate suppression were observed by the tumorigenic Jurkat cells. 5A. Simultaneously, no significant cytostatic (cells counted after 72 hours of incubation). 5B, and no cytotoxic effects (percentage of dead cells) after 72 hours of incubation). 5C was observed under dose-dependent conditions. Although initially (up to 24 hours), no reduced inhibitory effect of both compounds can be observed, the steadily increasing lactate formation in later incubation phases indicated an instability of the compounds. The data represents three independent measurements and are given mean ± SD (n = 3).