Small-molecule inhibitors of 6-phosphofructo-1-kinase simultaneously suppress lactate and superoxide generation in cancer cells
Fig 2
Preliminary screening of selected compounds for suppressing lactate and superoxide formation in Jurkat cells.
First, all 33 commercially available compounds selected by screening were examined to determine whether they suppress the formation of lactate and mitochondrial superoxide (SOX) by Jurkat cells. After 36 hours of incubation, statistically significant differences were observed between untreated (vehicle) and treated cells exposed to 50 µM of each inhibitor. In the Jurkat cells, four cmpds were found to suppress lactate (P* < 0.005), compared to the vehicle. However, the lowest level of significant difference in lactate suppression between treated and untreated cells was observed by cmpd No. 9, (P** < 0.001). By measuring SOX suppression, cmpd No. 30 proved to be the most successful (P** < 0.001), while cmpds No. 3 and 9 showed slightly higher values (P* < 0.005), compared to the vehicle. All compounds that showed a more potent inhibitory effect on a tumorigenic cell are marked in red.