Comparison of accumulation and distribution of PEGylated and CD-47-functionalized magnetic nanoporous silica nanoparticles in an in vivo mouse model of implant infection
Fig 5
Exemplary fluorescent microscopy images of magnetic implants, fluorescence score values for the tissue surrounding the implants and percentage of red fluorescent area of the total implant surface.
Implant of an animal from the CD-47 group (a), enlarged section of (a) with green autofluorescent material (b, asterisks) and red fluorescent nanoparticles (b, arrows); magnetic implant of an animal from the PEG-group (c); titanium implant of an animal from the CD-47 group (d); titanium implant of an animal from the PEG-group (e). Varying parts of the implants are covered with autofluorescent material (asterisks) which resulted in exclusion of some implant surfaces, the quantity of nanoparticles differs on the different implant materials and for the different nanoparticle types but without statistical significance, scale bar = 500 µm. Box and whisker plots of fluorescence score values for the tissue surrounding the implants (f) and the percentage of red fluorescent area of the total implant surface (g) for the two groups and the different implant materials (left = magnetic implant, right = non-magnetic implant). The boxes represent the 25th to 75th percentiles, the solid line indicates the median, triangles mark values outside the inner fences, CD-47 group n = 14 (both sides), PEG-group n = 13 (left) and n = 12 (right).