Perish the thawed? EDTA reduces DNA degradation during extraction from frozen tissue
Fig 2
Percentage of high molecular weight DNA.
Average values for percentage of high molecular weight DNA (%HMW) were determined for extracts prepared from frozen tissue samples collected from each of 10 individuals of 10 marine species (five marine fishes and five marine invertebrates). One sample from each individual was then thawed in EDTA (250 mM, pH 10; maroon) overnight at 4°C and the second was thawed in ethanol (95%; blue) overnight at 4°C, after which DNA was extracted, analyzed, and compared to DNA extracted directly from the third frozen tissue sample, which did not receive liquid preservative treatment (yellow). Error bars represent standard error. Within each histogram, treatments bearing different lower-case letters are significantly different at p < 0.05; matching lower-case letters indicate statistically indistinguishable treatments; an absence of letters indicates no significant differences among all treatments in a given model.