The neuronal chaperone proSAAS is highly expressed in the retina
Fig 5
Image quantitation of RGCs reveals differences in mouse proSAAS distribution as compared to bioinformatics analysis.
Panel A: Quantitative image analysis of retinal cross sections labeled for proSAAS (LS46 antiserum) in cell-marker positive cells (GFAP, n = 4; calbindin, n = 3; CHaT, n = 6; RBPMS n = 4; Brn3a n = 4). Panel B: Quantitative image analysis of 6 retinal cross-sections, colabeled with proSAAS, Brn3a, and melanopsin antisera, showing no significant differences. Panel C: Representative image of proSAAS, melanopsin, and Brn3a staining. (ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.0001, **** p < 0.00001, using one-way ANOVA with Tukey’s post-test for Panel A; and unpaired Student’s t-test for Panel B.). For antiserum compatibility purposes, Abcepta ALS16157 goat anti-proSAAS antibody was used for the experiments in Panels B and C.