Curcumin ameliorates aging-induced blood-testis barrier disruption by regulating AMPK/mTOR mediated autophagy
Fig 6
Activation of the AMPK/mTOR signaling pathway correlates with curcumin-activated autophagy to restore D-gal-induced tight junction damage in SCs.
(A) Protein expression and quantification of p -AMPK/AMPK and p-mTOR/mTOR after co-treatment of SCs with different concentrations of curcumin (0, 5, 10, and 20 μM) and 40 mg/mL D-gal by Western blotting. (B) Protein expression and quantification of Beclin1, LC3, Claudin-4, Claudin-7, Occludin, and ZO-1 in SCs after treatment with D-gal (40 mg/mL) and curcumin (20 µM)/CC (10 µM) by Western blotting. (C) Cellular immunofluorescence assay (scale bar = 50 μm) for protein expression and quantification of Beclin1, LC3, Claudin-4, Claudin-7, Occludin, and ZO-1 in SCs after D-gal (40 mg/mL) and curcumin (20 µM)/CC (10 µM) treatment. Data is presented as the mean ±standard error of the mean (SEM). The data are statistically significant between different letters (p<0.05).