Curcumin ameliorates aging-induced blood-testis barrier disruption by regulating AMPK/mTOR mediated autophagy
Fig 2
Curcumin restores D-gal-induced SCs TJ damage in vitro.
(A) The survival rate of SCs was determined after exposure to curcumin at concentrations of 0, 5, 10, 20, and 40 μM. (B) SCs were incubated with curcumin at concentrations of 0, 5, 10, and 20 μM in the presence of 40 mg/mL D-gal for 48 h, followed by Western blotting analysis to evaluate the expressions of Claudin-4, Claudin-7, ZO-1, and Occludin proteins. (C) A cellular immunofluorescence assay (scale bar = 50 μm) was performed to assess and quantify the expressions of Claudin-4, Claudin-7, Occludin, and ZO-1 in SCs co-treated with curcumin at concentrations of 0, 5, 10, and 20 μM and 40 mg/mL D-gal. Data is presented as the mean ±standard error of the mean (SEM). The data are statistically significant between different letters(p<0.05).