Photobiomodulation therapy increases neural stem cell pool in aged 3xTg-AD mice
Fig 2
PBM does not affect the rate of neurogenesis in aged 3xTg mice.
A. Representative images of DCX (red) and calretinin (CR, green) stained dentate gyrus sections showing DCX+ cells (arrowheads) and CR+ cells in the subgranular zone and granule cell layer (Scale bars = 100µm) B. Quantification of DCX and CR staining showing significant reductions in the number of newborn neurons in both 3xTg groups compared to WT with no effect of PBM. (One-way ANOVA. *p<0.05, **p<0.01, ***p<0.001). C. Analysis of newborn granule neurons at different stages of development shows a dramatic decrease in newly generated DCX+ neural progenitors and an overrepresentation of DCX-/CR+ immature neurons. D. Schematic representation of neurogenesis and timeline of marker expression from NSC (left) to mature granule neuron (right).