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Effective polyclonal antibodies against the virulence-associated protein D (VapD) of Helicobacter pylori, obtained from recombinant VapD

Fig 2

Expression of rVapD in E. coli Rosetta (DE3).

Aliquots of 20 µl of culture were collected at 0, 1, 2, 3, and 4 h after the addition of 0.1mM IPTG to verify the correct expression of rVapD by SDS-PAGE. MW, molecular weight.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0321455.g002