Matrix metalloproteinase-7 is dispensable in a mouse model of sepsis-induced acute lung injury
Fig 5
Histological Lung Injury induced by a septic insult [cecal slurry (CS; 1.6mg/g) + hyperoxia (HO; 95% O2)] in MMP7KO mice and their wild-type littermates.
Representative whole lung sections in mice used for histology scoring (A). Lungs were stained with H&E and 20x images of the entire section scanned by the Vanderbilt University Medical Center Digital Histology Shared Resource using the Leica SCN400 Slide Scanner. Septic mice (WT and MMP7KO) had numerically higher total lung injury scores compared to their respective controls (B). No differences were observed between WT and MMP7KO mice in either treatment group. Individual parameters of lung histology injury score are presented as a table (C). N = 3-10. [Statistical analysis: (B) Kruskal-Wallis test with a Dunn’s multiple comparisons test; (C) Mann-Whitney U test]. Each point represents an individual male (solid circle) or female (open circle) animal. Horizontal line indicates combined median of male and female animals. Control = 5% dextrose + room air at 21% O2. RBC = red blood cell, IQR = interquartile range.