Rapidly obtaining genome sequence of Severe Fever with Thrombocytopenia Syndrome virus directly from clinical serum specimen using long amplicon based nanopore sequencing workflow
Fig 5
Distribution of unidentical sites from Sanger sequencing.
A. A total of 10 unidentical sites between Sanger sequencing and nanopore sequencing distributed over the simulated sample whole genome. B. For M segment, 81, 408 and 2477 positions were in homopolymerize regions. For L segment, site 3089 was in the poly A region. C. Except homopolymer sites, all other unidentical sites’ second most base was identical with the Sanger sequencing.