Engineering the green algae Chlamydomonas incerta for recombinant protein production
Fig 5
Hybridizations of C. incerta holding bleomycin resistance and C. reinhardtii holding hygromycin B resistance.
A) Hybridizations were performed and 129 colonies were obtained on TAP agar plates containing 15 μg/mL zeocin and 30 μg/mL hygromycin. B) Four hybrids (D11, D9, E2, C10) that exhibited resistance to zeocin and hygromycin (and the parent strains) were plated onto TAP agar plates containing only 15 μg/mL zeocin, only 30 μg/mL hygromycin, or both 15 μg/mL zeocin and 30 μg/mL hygromycin. C) PCR analyses for the presence of bleomycin and hygromycin B resistance genes in the four hybrids, but amplicons for only 3 hybrids (D11, D9, C10) were obtained. The expected sizes of the PCR products were 500 bps for bleomycin and 162 bps for hygromycin B.