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Restorative effects of Momordica charantia extract on cerebellar GFAP and NGF expression in pregnant diabetic rats and their offspring

Fig 9

Electron micrographs of cerebellar cortex sections from diabetic mothers (I–L).

Cerebellar cortex sections from diabetic mothers showing, (I): reveals apoptotic Purkinje cell with indefinite nucleus and dilation in (rer) and dark lysosomal patches (L), swallowed mitochondria (m) could be noticed. (G–L): showing granular layer suffering from neuropil degenerative changes (blue arrow), abnormal granulocytes (Gc) and microglial cells (Mg), apoptotic bodied with pyknotic nuclei (red arrows) and dilated neural axons (*). Astrocyte of fibre-type (Ac) could be distinguished. (M–P) Cerebellar cortex sections from DM+BM group showing, (M): Combined treatment shows quite normal Purkinje cell with a typical eukaryotic nucleus (N) and a discernible nucleolus (nu), its cytoplasm is surrounded by nerve fibers (nf), mitochondria (m), and rough endoplasmic reticulum (rer). (N–P): showing the granular layer with a complete neuropil comprising granulocytes (Gc), microglial cells (Mg), myelinated neural axons (*) that have formed synapses, (green arrow) Denotes the multivesicular body of the neuropil.

Fig 9

doi: https://doi.org/10.1371/journal.pone.0321022.g009