Restorative effects of Momordica charantia extract on cerebellar GFAP and NGF expression in pregnant diabetic rats and their offspring
Fig 5
Photomicrographs of H&E-stained cerebellum sections from control and different offspring’s’ groups.
Control (A&B) and BM (C&D) groups, showing the normal four-layered structure of the cerebellar cortex; the external granular layer (EG), the molecular layer (M), the Purkinje cell layer (P) and the internal granular layer (IG). (E&F) Cerebellar cortex sections from diabetic mothers showing Focal loss of Purkinje cells leaving empty baskets (green arrow), marked necrosis with hypereosinophilic, shrunken, angular neuroplasm and nuclear pyknosis (red arrow). EG: external granular layer, M: molecular layer, P: Purkinje cell layer and IG: internal granular layer. (G & H) Cerebellar cortex sections from DM+BM group showing quite normal external and internal granular cell layer (EG&IG), molecular layer (M), while the Purkinje cell layer (P) displayed a few numbers of degenerated cells with pyknotic nuclei (red arrow). (I): represents approximate Purkinje cells number in different experimental groups. Right panel 100X, left panel 400X.