Solute carrier family 2 member 2 (glucose transporter 2): a common factor of hepatocyte and hepatocellular carcinoma differentiation
Fig 5
The IGF1R pathway is upregulated following slc2a2 knockdown in HepG2 cells and zebrafish embryos.
(A) qPCR analysis of IGF1R and IGF2 gene expression in HepG2 cells. Knockdown of slc2a2 using shGLUT2 significantly increased IGF1R and IGF2 expression compared to the pLKO control group (p < 0.05). Overexpression of slc2a2 (GLUT2 over) significantly decreased IGF1R and IGF2 expression compared to the pMSCV control group (p < 0.01 for IGF1R, p < 0.05 for IGF2). (B) qPCR analysis of igf1r mRNA expression in zebrafish embryos at 2 dpf. slc2a2 knockdown significantly increased igf1r expression compared to the control group (p < 0.001). (C) Immunohistochemistry (IHC) staining for IGF1R protein in zebrafish embryos at 2 dpf. slc2a2-MO treated embryos showed stronger IGF1R fluorescence intensity compared to control embryos, indicating increased IGF1R protein expression. All analyzed embryos (11/11) showed consistent results in both groups.