TWINKLE: An open-source two-photon microscope for teaching and research
Fig 9
Examples for teaching principles of two-photon microscopy.
A) Transmitted laser power as function of the control voltage applied to the Pockels crystal for two different crystal orientations. The red orientation is far from the optimum and produces a small dynamic range for intensity control. The blue orientation is close to the optimal. The thin purple line is a best fit (see text). B) Fluorescence in Fluorescein as a function of applied dispersion compensation. A fit allows to estimate the pulse width. Note also the symmetric shoulders not captured by the fit, suggesting a non-Gaussian pulse shape.