TWINKLE: An open-source two-photon microscope for teaching and research
Fig 8
Example calcium imaging in adult Drosophila melanogaster.
A) Imaging from the brain of a head-fixed adult fly [38]. A dissection is performed to remove the cuticle from the back of the fly’s head and provide optical access to the brain, and the imaged brain volume is outlined. The transgenic flies express pan-neuronal GCaMP6s and myr-tdTomato, driven by the nsyb promoter. B) Average projections over ten 5 μm-spaced planes of both imaging channels, acquired at a temporal resolution of 1 volume/s. XY boundaries of exemplar ROIs are indicated. C) The channels are motion corrected and spatially clustered ROIs are extracted. Examples of spontaneous calcium activity extracted from ROIs in the brain volume.