TWINKLE: An open-source two-photon microscope for teaching and research
Fig 6
Example imaging data from simple samples.
A) Autofluorescence of a part of a dandilion flower (Taraxacum officinale) in vivo. Notice the pollen grains embedded in the plant material. Optical sectioning allows to focus below the plant surface to the grains. B) Zoom and volumetric image of a small region (white box) of the sample in A. Optical sectioning shows the surface of the plant and the slice where the pollen grains come into focus. Notice the complex 3D structure of the pollen grains, and their embedding below a layer of red-fluorescent plant material. C) Histological sample showing a stain for glial fibrillary acidic protein in rat astrocytes. D) Image of a cotton napkin (Kimwipe) with depth coded as color. Zooming into a small region (white box) shows how strands of cotton are woven together, and how the pattern changes with depth. Notice how image quality deteriorates in this strongly scattering sample.