Chemical nucleases are a robust alternative for RNase H cleavage of human ribosomal RNA
Fig 2
The artificial nuclease (ARRR) cleaves 28 S rRNA into two fragments.
A Using ARRR, the DNA-catalyst conjugate 1v splits the 28 S rRNA into fragments of approximately 4,000 and 1,200 nucleotides, in contrast to the unconjugated probe 2v. RNase H digestion yields identical fragments with both probes. RNAs were separated on an 2% Agarose-TAE Gel. Stained with GelRed. B DNA probe 1v and 2v target sequence and cleaving site on the human 28 S rRNA.