Extant life detection using label-free video microscopy in analog aquatic environments
Fig 10
Helens glacial cave ice samples. (A, B) MHI images showing motion of a single recording conducted in situ immediately after ice melted. All motile small microbes in glacial ice samples had low SNR and were therefore difficult to identify in MHI. Arrows in each image indicate an observable trajectory. Both MHIs were created using reconstructed amplitude images where each image in the time series was the z-plane where the microbe of interest was in focus. This approach allowed the MHI to detect these two trajectories. (C) MATLAB plot showing the corkscrew trajectory of the motile small microbe shown in the bottom left of (A), (D). (D) MATLAB plot showing manually tracked 2D trajectories of all six motile small microbes observed in this recording.