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Inhibition of CFTR-mediated intestinal chloride secretion by nornidulin: Cellular mechanisms and anti-secretory efficacy in human intestinal epithelial cells and human colonoids

Fig 5

The effect of nornidulin on intracellular cAMP level.

The experiment involved pretreating T84 cells for one hour with different conditions: vehicle (control), nornidulin, N9 (5 μM), nornidulin (5 μM) with FSK (5 μM), or FSK (5 μM). Following treatment, intracellular cAMP levels were determined using a cAMP assay kit. The results are expressed as cAMP concentrations with standard deviation (S.D.) (n = 5–6). ns, not significantly different from the control group. **, p <0.01 compared to the indicated group. ****, p <0.0001 compared to the indicated group.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0314723.g005