Inhibition of CFTR-mediated intestinal chloride secretion by nornidulin: Cellular mechanisms and anti-secretory efficacy in human intestinal epithelial cells and human colonoids
Fig 2
Modulation of cAMP-mediated Cl− efflux and cell viability by nornudulin in T84 cells.
(A) Demonstrates nornidulin’s impact on cAMP-mediated Cl- efflux stimulated by 20 μM forskolin (FSK). Various concentrations of nornidulin were applied to the apical solutions (n = 5). The inset graph shows nornidulin’s effect on basal current at different concentrations (n = 5). (B) Cell viability after 24-hour exposure to varying concentrations of nornidulin in apical solutions (n = 5). A 10% DMSO solution serves as the positive control. Results are presented as percentage of cell viability relative to the control ± standard deviation (S.D.). ****, p<0.0001 compared to control, while "ns" denotes non-significant differences from the control.