Dual inhibition of oxidative phosphorylation and glycolysis exerts a synergistic antitumor effect on colorectal and gastric cancer by creating energy depletion and preventing metabolic switch
Fig 3
Effect of metabolic changes on tumor microenvironment.
(A)The pH of the medium 4h after treatment (NCI-006 [1 μM] and/or IACS-010759 [1 μM]) was measured to evaluate pH changes in vitro. The pH changes in vivo were measured by homogenizing tumors from carcinoma-bearing mice 4 h after treatment and dilution with ultrapure water. Data are presented as means ± SEM (n = 3 for each group, *p < 0.05, **p < 0.01, t-test). (B)Oxidative stress in the HCT116 xenografts. Mice were injected with ROS Brite 700 nm dye 4 h after the administration of NCI-006 and/or IACS-010759, and 20 min later, the tumors were removed, and fluorescence imaging was immediately performed. Data are displayed as the means ± SEM (n = 4 for each group, *p < 0.05, t-test).