Interplay between de novo and salvage pathways of GDP-fucose synthesis
Fig 7
Influence of TSTA3, GMDS and FCSK protein inactivation on the level of proteins involved in fucose recycling in mammalian cells.
(A) and (B) wild-type, TSTA3KO and GMDSKO HEK293T cells fed and not fed with fucose as well as FCSKKO cells were analysed by western blotting to determine protein levels of FUOM; as a loading control, an anti-calnexin antibody and anti-HSP60 antibody were used. (C) and (D) western blotting of FUCA1 performed on wild-type, TSTA3KO, GMDSKO, supplemented and unsupplemented in fucose and FCSKKO cell lysates. Anti-HSP60 and anti-calnexin antibodies were used as a loading control. For (A) and (D), ns, not significant as determined using one-way ANOVA with the Tukey post-hoc test. For (B) and (C), ns, not significant as determined using Welch’s t-test. Data are represented as mean ± SEM. Each sample was run at least in two biological replicates.