Interplay between de novo and salvage pathways of GDP-fucose synthesis
Fig 4
Changes in levels of proteins involved in GDP-fucose synthesis.
(A) FPGT western blotting analysis of the wild-type, TSTA3KO and GMDSKO HEK293T cells without and after supplementation with fucose. As a loading control, the anti-GAPDH antibody was used. (B) FCSK western blotting analysis of the wild-type, TSTA3KO and GMDSKO HEK293T cells after supplementation with or without fucose. As a loading control, the anti-GAPDH antibody was used. The main change in FCSK protein level in TSTA3KO cell line was indicated with a green box. (C) GMDS western blotting analysis of the wild-type and FCSKKO HEK293T cells. As a loading control, the anti-GAPDH antibody was used. (D) TSTA3 western blotting analysis of the wild-type and FCSKKO HEK293T cells. As a loading control, the anti-GAPDH antibody was used. The main change in TSTA3 protein level in FCSKKO cell line was indicated with a green box. For (A) and (B), ns, not significant; *, p < 0.05 as determined using one-way ANOVA with the Tukey post-hoc test. P values that trend to be statistically significant are also shown. For (C) and (D), ns, not significant; *, p < 0.05 as determined using Welch’s t-test. Data are represented as mean ± SEM. Each sample was run at least in two biological replicates.