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Interplay between de novo and salvage pathways of GDP-fucose synthesis

Fig 3

Analysis of the effect of supplementation of the TSTA3KO and the GMDSKO HEK293T cell lines with different fucose concentrations.

(A) quantification of intracellular GDP-fucose concentration. (B) quantification of the percentage of the core-fucosylated N-glycan structures; each sample was run in three biological replicates. Data are represented as mean ± SEM. Each sample was run in three biological replicates, ns, not significant; **p < 0.01; and ****p < 0.0001, as determined using one-way ANOVA with the Tukey post hoc test.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0309450.g003