Interplay between de novo and salvage pathways of GDP-fucose synthesis
Fig 2
Analysis of intracellular concentration of GDP-fucose and the level of fucosylated structures in the control and fucose-fed knockouts generated in the HEK293T cell line.
(A) and (B) present intracellular concentration of GDP-fucose and the level of fucosylated structures in the TSTA3KO cell line, respectively. (C) and (D) present the intracellular concentration of GDP-fucose and the level of fucosylated structures in the GMDSKO cell line, respectively. (E) and (F) show the intracellular concentration of GDP-fucose and the level of fucosylated structures in the FCSKKO cell line, respectively. Data are presented as mean ± SEM. Each sample was run in three biological replicates, ns, not significant and ****, p < 0.0001, as determined using one-way ANOVA with the Tukey post hoc test.