A combination of topical and systemic administration of brimonidine is neuroprotective in the murine optic nerve crush model
Fig 2
Sampling method for cell counting in retinal whole-mounts.
DAPI-, NeuN-, and GFAP-positive cells were counted in 15 randomly chosen sampling boxes (0.04 mm2): the central (n = 5, red squares), mid-periphery (n = 5, orange squares), and peripheral (n = 5, yellow squares) regions of the retina. DAPI, 4′,6-diamidino-2-phenylindole; NeuN, neuronal nuclei; GFAP, glial fibrillary acidic protein. Scale bar 1000 μm.