Development of a reliable, sensitive, and convenient assay for the discovery of new eIF5A hypusination inhibitors
Fig 5
GC7 analogs are hypusination inhibitors.
(A) Hyp’Assay: Dose-response of GC7 analogs (1, 2, 3 and 4) from 0 to 1 mM in the presence of 5 μM of spermidine. (B) MCF7 or (C) HT29 were treated with 40 μM of GC7, 1, 2, 3 or 4 for 24 h. Cells were lysed and analyzed by Western blot using the indicated antibodies. A quantification of 4 and 5 experiments is provided.