Characterizing primary transcriptional responses to short term heat shock in Down syndrome
Fig 5
Single Cell RNA-seq indicates that changes in heat shock-induced gene expression in trisomy 21 cells are population-wide.
A) Violin plots of percent of total normalized scRNA-seq gene counts per cell for two genes (CCT8, SUMO3) present on chromosome 21 and non-responsive to heat shock. B) Heatmap of the Z-scores of chromosome 21 genes showing increased reads in trisomy 21 cells. C) Violin plots of two heat shock responsive genes (HSPD1, HSP90AA1) not encoded on chromosome 21; the y-axis is the levels across more than 500 cells in each sample via scRNA-seq. D) Heatmap of the Z-scores of heat shock genes shows a general up-regulation in the expression of heat shock genes, rather than a few cells with extreme heat shock phenotypes. Violin plots showing the E) sum and F) median of Z-scores of all heat shock genes for more than 500 cells in each sample. The genes showed must be present in at least 75% of cells. All panels: control (light color) and heat shock (dark color) of disomic (green) and trisomic (purple) lymphoblastoid cells.