Heterogeneity in establishment of polyethylene glycol-mediated plasmid transformations for five forest pathogenic Phytophthora species
Fig 5
Comparison of Phytophthora sojae and P. ramorum putative transformant mycelial growth.
Putative transformants (PTs) of the control species Phytophthora sojae P6497 (A) and P. ramorum NA2_17 (B) growing on V8 agar supplemented with 50 μg/mL G418 and 30 μg/mL G418, respectively, four days post transformation. While putative P. ramorum transformants did grow on three of the plates, the mycelial growth was weak and irregular (see arrows) relative to the P. sojae control. The white arrows indicate PTs that would have been considered “strong” for P. ramorum, whereas the grey arrows indicate very weak PTs. All P. ramorum PTs were taken through a second round of selection, but most did not grow at the increased concentration of G418; none of the “weak” PTs (grey arrows) grew, and only two of the “strong” PTs (white arrows) grew. The same pattern emerged for every P. ramorum transformation attempted.