Orexin receptor 2 agonist activates diaphragm and genioglossus muscle through stimulating inspiratory neurons in the pre-Bötzinger complex, and phrenic and hypoglossal motoneurons in rodents
Fig 5
Intravenous (i.v.) OX-201 administration significantly increased burst frequency of the diaphragm in anesthetized rats.
(A) Representative traces by EMG recording of the diaphragm before and after administration of vehicle or OX-201 at 3 mg/kg. (B) The method to calculate burst amplitude and tonic activity from integrated EMG signals. (C) Effects of OX-201 on burst frequency, burst amplitude, and tonic activity of the diaphragm. Tonic activity was defined as the mean value of integrated EMG signals between bursts. Mean values in burst frequency, burst amplitude, and tonic activity during 2 min before vehicle or OX-201 administration were used as control values, and those during 10 min after vehicle or OX-201 administration were used to calculate percent changes from control values. n = 8, *P = 0.040, ***P < 0.001 versus vehicle-treated rats. Data are presented as mean ± standard error of the mean.