A homogeneous time-resolved fluorescence screen to identify SIRT2 deacetylase and defatty-acylase inhibitors
Fig 5
The ability of 15 small molecules to bind SIRT2 or affect its deacetylase or demyristoylase activities.
“N.D.” means not determined, and “no effect” indicates that 200 μM compound did not significantly affect SIRT2 activity. The Kd value for 1 was determined from a Cy3-myristoyl-H4K16 peptide binding competition assay. The Kd values for 2, 4, 5, and 6 were determined from a 1-aminoanthracene binding competition assay. *All data for 3 was previously published [12]. See Materials and Methods for experimental details and the names of the chemicals.