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Vorolanib, sunitinib, and axitinib: A comparative study of vascular endothelial growth factor receptor inhibitors and their anti-angiogenic effects

Fig 4

HUVEC sprouting assay results.

(A) Representative photos of the spheroids when treated with two different concentrations of each TKI. (B) Quantitative analysis of the cumulative sprout length per spheroid (CSL) for each TKI at the different test concentrations. Results are (mean ± SD). Controls were ± 25 ng/mL VEGF-A. Results are (mean ± SD). Statistical differences were made visible by the presence of stars: *0.05 ≥ p > 0.01; **0.01 ≥ p > 0.001; ***0.001 ≥ p ≥ 0.0001; ****0.0001 ≥ p. CSL, cumulative sprout length; HUVEC, human umbilical vein endothelial cell; SD, standard deviation; TKI, tyrosine kinase inhibitor; VEGF, vascular endothelial growth factor.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0304782.g004