The interplay of Pseudomonas aeruginosa and Staphylococcus aureus in dual-species biofilms impacts development, antibiotic resistance and virulence of biofilms in in vitro wound infection models
Fig 5
Raman analysis of the penetration ability of gentamicin into the biofilms.
Fixed mono- and dual-species biofilms were incubated with a 100 mg/mL gentamicin solution. For every Raman spectrum, the area under the curve (AUC) of a distinct gentamicin peak was determined and the ratio between the gentamicin applied and the AUC of the gentamicin in the biofilm was calculated.