Custom-made 3D-printed boot as a model of disuse-induced atrophy in murine skeletal muscle
Fig 5
Effects of 1 week immobilization.
(A) Quantitative RT-PCR of NJM genes expression in immobilized, free, and free roaming wild type leg after one week of immobilization, TBP normalized. (B) Comparison between free, immobilized, and free-roaming wild type legs of gastrocnemius muscle wet weight, (C) cross-sectional area and (D) relative fiber size distribution, and (E) Quantitative RT-PCR of atrogenes expression, TBP normalized (n = 5). Statistical significance was calculated using two-tailed Student’s t test. Data are mean±SEM (A, B, C, E), mean±SD (D). * p<0,05 ** p<0,01 *** p<0,001.