Itaconic acid inhibits nontuberculous mycobacterial growth in pH dependent manner while 4-octyl-itaconic acid enhances THP-1 clearance of nontuberculous mycobacteria in vitro
Fig 5
pH neutral itaconic acid does not inhibit the growth of M. abscessus and M. avium.
Clinically isolated (A) M. abscessus 253 and (B) M. avium 813 was grown in 7H9 minimal media supplemented with 10 mM SCFAs: Acetate, propionate, and butyrate. IA (pH≈7) was added at the indicated concentrations and an OD600 reading was taken each day to track bacterial growth. Statistical tests were performed using Two-Way ANOVA; Tukey’s multiple comparison test, on log transformed data. Error bars indicate standard deviation and significance indicates where the SCFA control was first found to be significantly different from 10 mM Itaconic acid samples. * p ≤ 0.05. ** p ≤ 0.005, ** p ≤ 0.0005, **** p ≤ 0.0001. n = 3–5.