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(S)-(-)-blebbistatin O-benzoate has the potential to improve atopic dermatitis symptoms in NC/Nga mice by upregulating epidermal barrier function and inhibiting type 2 alarmin cytokine induction

Fig 3

(S)-(-)-blebbistatin O-benzoate reduces mast cell infiltration in AD-like lesions of NC/Nga mice.

(A) Toluidine blue staining of auricular tissue of vehicle and (S)-(-)-blebbistatin O-benzoate on Day 35. Scale bar = 50 μm. (B) The number of mast cells stained with toluidine blue was measured and quantitatively evaluated in six areas from each tissue (n = 8 per group). (S)-(-)-blebbistatin O-benzoate treatment significantly decreased mast cell numbers. Data represent mean ± SE, *p < 0.05 vs. vehicle.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0302781.g003