Anti-inflammatory effect of the combined treatment of LMT-28 and kaempferol in a collagen-induced arthritis mouse model
Fig 3
Combination effect of LMT-28 and kaempferol on RANKL-induced osteoclastogenesis.
BMMs isolated from normal mice were stimulated with M-CSF (20 ng/mL) and/or RANKL (40 ng/mL) and treated with LMT-28 (25 μM) and/or kaempferol (12.5 μM). (A) Microscopic image (200× magnification) of osteoclasts stained for TRAP activity and (B) quantification of the number of TRAP+ multinucleated osteoclasts (≥3 nuclei) in each treatment group are presented. (C) The mRNA levels of c-fos, nfatc1, ctsk and trap in osteoclasts were measured. Data are presented as mean ± SEM. #p<0.05 vs. M-CSF-treated group; *p<0.05, **p<0.01, and ***p<0.005 vs. M-CSF- and RANKL-treated control group.